International journal of research in pharmaceutical and. International journal of research in pharmaceutical and nano sciences. Preparation and evaluation of chlorpheniramine maleate. Comparisonof emulsification and ionic gelation method of.
Production and characterization of nanocapsules encapsulated. In this paper, as an active ingredient, puerarin chitosan nanoparticles purcstppnps are prepared by an ionic gelation method. Understanding the mechanism of ionic gelation for synthesis. Thus, a simple liquid will not typically support a transverse acoustic phonon, or shear wave. Ionotropic gelation is based on the ability of polyelectrolytes counter ions to cross link to form hydrogels. Synthesis and characterization of new thiolated chitosan. Ionotropic gelation a novel method to prepare chitosan nanoparticles article pdf available in research journal of pharmacy and technology 44. We derivatized low molecular weight chitosan lmwc with 3mercaptopropanoic acid 3mpa by a coupling reaction. One percent wv sodium nitrite solution was used to digest the cs polymer into oligomers and monomers, and incubated for one week in a shaking water bath 37c. The shellforming material represents at least 10% of the weight of the dry. In one of the methods, the cross linker ion is positioned externally shown in fig 2. Ionotropic gelation is based on the capability of polyelectrolytes to traverse link in the presence of counter ions to form nanoparticles giri et al.
Biological activities of chitosan and prepared chitosan. When an increase in the polymer concentration retards the drug release more than 12 h. The molecular interactions of the ionic crosslinking of chitosan with tripolyphosphate have been investigated and elucidated by means of allelectron density functional theory. Preparation of chlorpheniramine maleateloaded alginatechitosan particulate systems by the ionic gelation method for taste masking mitra jelvehgari 1,2. Preparation of chlorpheniramine maleateloaded alginate. The drug nanoparticles were prepared using chitosan polymer by ionic gelation technique. The possibility of three kinds of anions tripolyphosphate, citrate and sulphate to interact with chitosan was investigated by turbidimetric titration.
The ability of such highly cross linked structure to sustain the drug release is exploited in this method. This study investigated the preparation of cstpp nanoparticles loaded with polyphenolic rose hips extract by ionotropic gelation method. Thus, numerous studies have highlighted the importance of size in nanoparticles synthetized from lmw chitosan by ionic gelation method. Ionotropic gelation an overview sciencedirect topics. Introduction gastric residence time grt is an important parameter which may affect drug bioavailability of dosage forms. Emulsification, ionic gelation method, mucoadhesive microsphere 1. Development and testing of novel temoxifen citrate loaded. In a static sense, the fundamental difference between a liquid and a solid is that the solid has elastic resistance against a shearing stress while a liquid does not. Pharmaceutics free fulltext preparation of puerarin. Encapsulation using ionic gelation can be used to mask the bitter taste of spirulina. Preparation of puerarin chitosan oral nanoparticles by ionic. The technique proposed is based on principle of ionotropic gelation polyelectrolyte complexation. The invention relates to a method for ionic gelation on solids for encapsulating waterinsoluble solids. Preparation and in vitro characterization of chitosan.
Chitosan nps solutions were prepared by mixing of the 10 ml chitosan solutions, with different concentrations 1, 2 and 3. Irshad alam 2, anupam k sachan 1 and sudhir s gangwar 3 1dayanand dinanath college, institute of pharmacy, kanpur, india. Ionotropic gelation method to achieve a pharmaceutical product with desired characteristics. These methods differ from each other in the source of the cross linking ion. Fabrication and evaluation of propranolol hydrochloride.
One of the biggest advantages of the alginate is in the form of a. Ionic gelation method was utilized to prepare the calcium alginate beads. Ionotropic gelation is based on the ability of polyelectrolytes counter ions to. Research article synthesis and characterization of new. Jan 22, 2016 chitosan nanoparticles were prepared from chitosan with various molecular weights by tripolyphosphate tpp ionic gelation combined with a spray drying method. Microspheres of etodolac using ionic gelation method ramya sri sura department of pharmacy, university of technology, osmania university, hyderabad 500007, telangana, india. Ionotropic gelation is based on the ability of polyelectrolytes to cross link in the presence of counter ions to form hydrogels. The using of ionic gelation method based on polysaccharides. Preparation and evaluation of mucoadhesive microspheres of. Microcapsules, orlistat, ionic gelation method, hpmc, carbopol940. The morphologies and characteristics of chitosan nanoparticles were determined by tem, fesem and from their mean sizes and zeta potentials.
Preparation of calcium alginatetetrandrine beads using ionic. Ionic gelation is based on electrostatic interactions between negatively charged functional groups that belong to the crosslinker agent. The molecular interactions of the ionic crosslinking of chitosan with tripolyphosphate have been investigated and elucidated by means of. As the linking continues, larger branched polymers are obtained and at a certain extent of the reaction links between the polymer result in the formation of a single. The results of fourier transformation infrared spectroscopy ftir showed that linalool was successfully encapsulated in the wall materials. As the linking continues, larger branched polymers are obtained and at a certain extent of the reaction links between the polymer result in the formation of a single macroscopic molecule. Ionotropic gelation technique for microencapsulation of. We researched the influence of 3mpa on the nanoparticles formation by ionic gelation method using sodium tripolyphosphate tpp as. Microencapsulation of lactobacillus plantarum dkl 109 using external ionic gelation method 693 the method used to form the beads. Simvastatin and all other polymers were individually passed through sieve no 60. Many drug suffer from low bioavailability because of short gastric empting time. Preparation of calcium alginatetetrandrine beads using.
Chitosan nanoparticles were prepared from chitosan with various molecular weights by tripolyphosphate tpp ionic gelation combined with a spray drying method. Since, the use of alginates, gellan gum, chitosan, and carboxymethyl cellulose for the encapsulation of drug and even cells, ionotropic gelation technique has been widely used for this purpose 11. Alginate is an anionic polysaccharide polymer extracted from seaweed. The purpose of this study is to formulation and evaluation of floating and mucoadhesion microspheres of etodolac using ionic gelation method. Moreover, it could efficiently retain the bioactivity of macromolecules such. The orders k a and p eff of purcstppnps prepared by the ionic gelation method were duodenum jejunum ileum colon, and, among them, the absorption of the duodenum, jejunum, and ileum in the lowdose group did not increase significantly, but there was a significant difference p method and emulsion twophase system, also called external ionic gelation and internal ionic gelation techniques, respectively krasaekoopt et al. Nanoparticles of different ratios were formulated and analyzed for drug content, entrapment efficiency, particle size, zeta potential and in vitro drug release studies. The best formula was coated with ph sensitive polymers, i. The nanoparticle can be made by the method of ionic gelation, which is a method that utilized the electrostatic interaction between cationic and anionic polymer that spontaneously from nanosized. The ability of such highly cross linked structure conditionto sustain the drug release is exploited in this method.
The chitosan cs concentration, ph of the cs solution, sodium tripolyphosphate tpp concentration, stirring speed, stirring time, ultrasonic power, and dosage are used as single factors for investigation, and the encapsulation efficiency, drug loading capacity. Formulation and characterization of chondroitin sulfate. Atorvastatin alginate beads were prepared by ionic gelation method by using different curing agents and studied the different process variable like the effect of alginate concentration, the effect of rpm and curing agents on drug entrapment and to improve the bioavailability of the drug. In the ionic gelation method, chitosan polysaccharide is dissolved in aqueous acidic solution to get the cation of chitosan. Ionic gelation controlled drug delivery systems for gastric. One method to prepared microcapsules is to use an ionic gelation method crosslinking between sodium alginate and calcium chloride 8. Gelation gel transition is the formation of a gel from a system with polymers. The ability of such highly cross linked structure to. Comparisonof emulsification and ionic gelation method of preparation of mucoadhesive microsphere swati chaturvedi1, prof. The advantage of this method was attributed to its mild conditions achieved without applying harmful organic solvent, heat or vigorous agitation that are damaging to sensitive proteins.
Orifice ionic gelation method for mucoadhesive microcapsules. Present study focused on the preparation of the chitosan nanoparticles nps throughout ionic gelation method and using tripolyphosphate tpp, as nontoxic polyanion counterions. Characterization of spirulinaalginate beads formed using. Sieving method is most widely used procedure to assess the particle size loyd et al. This work reports details pertaining to the formation of chitosan nanoparticles that we prepare by the ionic gelation method. Fabrication and evaluation of propranolol hydrochloride loaded microspheres by ionicgelation technique nazia khanam 1, md. Ionic gelation is a chemical reaction between sodium alginate and calcium chloride, where the sodium ions are replaced by the calcium ions to form a gellike structure. Predictive tools for achieving the sustained action of pioglitazone hydrochloride.
Farhad barghi 3 1 drug applied research center, tabriz university of medical sciences, tabriz, ir iran 2 faculty of pharmacy, tabriz university of medical sciences, tabriz. The mucoadhesive property of the microcapsules was evaluated by an in vitro adhesion testing method, known as wash off method. There are two methods by which hydrogel beads can be generated using ionotropic gelation technique. Introduction oral drug delivery system is often more susceptible route of administration than other routes because physiology of gi tract offers more flexibility in dosage regimen gupta and robinson, 1992. Micropellets of verapamil hydrochloride were formulated by ionotropic gelation technique using sodium alginate, hydroxy propyl methyl cellulose and hydroxy propyl cellulose. Microcapsules, chlorpheniramine maleate, ionic gelation method, invitro release. This technique involves interaction of a cation or an anion with an ionic polymer to generate a highly cross linked structure. The effects of different preparation conditions on.
Department of pharmaceutical technology, meerut institute of engineering and technology, nh58, baghpat bypass crossing, delhiharidwar highway, meerut250005 up. Insight on the formation of chitosan nanoparticles through. Branched polymers can form links between the chains, which lead to progressively larger polymers. The chemical modification of lmwc was characterized by fourier transform infrared spectroscopy ftir and nuclear magnetic resonance, 1 hnmr. Since, the use of alginates, gellan gum, chitosan, and carboxymethyl cellulose for the encapsulation of drug and even cells, ionotropic gelation technique has. Calcium pectinate beads were prepared by ionic gelation method with varied calcium chloride concentration 5%, 10%, and 15%. Preparation of chitosan nanoparticles by tpp ionic. The compositions of different formulations are given in table no. The method uses negatively charged macromolecules and soluble salts of polyvalent cations as shellforming materials having a high adsorption on the surface of the solid when subjected to thermal treatment.
Preparation and evaluation of chlorpheniramine maleate microcapsules by ionicgelation method. Microencapsulation of lactobacillus plantarum dkl 109 using. Ramdhani d 2017 formulation and characterization of chondroitin sulfate nanoparticle with chitosan as polymer and kappa carrageenan as crosslinker using the ionic gelation method. Catechin, with its low stability in alkaline conditions, was chosen as a candidate antioxidant to be used in the ionic gelation procedure by dube et al. Development and testing of novel temoxifen citrate loaded chitosan nanoparticles using ionic gelation method paresh n. International journal of research pharmaceutical and nano. Chitosan is a cationic natural polymer which has been widely used in various areas. Solvent effects have been taken into account using implicit models. Sodium alginate solution 2% wv in deionized water was mixed with tetrandrine solution in hcl 0.
Scanning electron microscopy sem results demonstrated that the shapes of linnanocapsules, with smooth surfaces, were nearly spherical. Atorvastatin alginate beads were prepared by ionic gelation method by using different curing agents and studied the different process variable like the effect of alginate concentration, the effect of rpm and curing agents on drug entrapment and to. Ionotropic gelation technique for microencapsulation of antihypertensive drug. The mucoadhesive property of microcapsules was compared with that of a. Chitosan microspheres were prepared by an emulsionphase separation technique without the use of chemical crosslinking agents. Preparation of chitosan nanoparticles by tpp ionic gelation. Ionic gelation controlled drug delivery systems for gastricmucoadhesive microcapsules of captopril. Especially, ph is most effective in the ionic gelation method.
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